Biomedical Optical Imaging: Biomedical Research Applications

Developing advanced biomedical imaging systems is our second focus in biomedical imaging. Our focus in this field is developing high throughput biomedical imaging systems with new optical systems, building on our unique capability in developing custom optical systems.

Currently we are developing array confocal fluorescence imaging system funded by NIH. This confocal system addresses the major limitation of small field of view in current focal imaging system. We have developed efficient design and analysis methods of grating beamsplitter for array imaging. Our methods were reported in two journal papers and two presentations. One more paper will be submitted for review soon. Working with Prof. Carol Barnes at Department of Psychology, Neurology and Neuroscience and Prof. Urs Utzinger at Department of Biomedical Engineering, we are developing large format confocal fluorescence microscope for brain imaging. This microscope will have large field of view (10x10mm), long working distance (>12mm), and high resolution (numerical aperture >0.5). With multifocal scanning capability, the imaging speed is much higher than conventional confocal fluorescence microscope. We will demonstrate the system in this fall semester and apply NIH funding for further development in 2016.

Collaborating with Dr. Stanley Pau at College of Optical Sciences and Dr. Guang Yao at Department of Molecular & Cellular Biology, we will develop advanced quantitative phase and polarization microscope. I am the lead PI for this NSF supported project. The proposed instrument operates at an acquisition speed that is at least an order of magnitude faster than existing microscopes. The performance of this multimodal imaging (spectral, polarization and phase) microscope will be evaluated by imaging of breast cancer cell lines and human cervical epithelial cell line, and will be tested in single-cell cancer pathway studies.