Research

Fourier multiplexed lifetime imaging (FmFLIM)

We invented a fast Fourier spectroscopic method that performs true parallel fluorescence lifetime imaging (FLIM) in all excitation-emission channels. The instrument simultaneously measures fluorescence lifetime and intensity at every excitation-emission combination within microseconds.

Confocal FmFLIM microscopy

We developed a hyper-spectral lifetime confocal microscope based on the FmFLIM method. The microscope handles multiple fluorescent labels in a single image.  The multi-channel-FLIM image allows fully quantitative analysis of multicolor Förster resonance energy transfer (FRET), which enables dynamic imaging of complex protein interaction and multiplexed FRET sensing. Sample images of the EEM-FLIM confocal microscope can be found in the “Lifetime Imaging” page.

Deep-tissue imaging with FmFLIM
We developed a scanning laser optical tomography (SLOT) system in order to perform FmFLIM imaging in deep tissue. The system will allow simultaneously imaging of multiple signaling events in live organisms. Movies of live zebrafish 3D lifetime imaging can be found in the “Lifetime Imaging” page.

Multi-photon light-sheet microscopy

 

Light-sheet microscopy achieves better imaging penetration by illuminating the sample from the side. We developed a two-photon light-sheet microscope (2p-LSM) with 1~2 micron axial resolution and 0.5 micron lateral resolution. The microscope has an >500 micron extended field view, and deep penetration in live tissue. We are applying the technique to brain imaging. Movies from zebrafish and mouse can be seen in the “Bessel Light Sheet Two-Photon Imaging” page.

 

Random depth-access light sheet microscopy

Random depth-access light sheet microscopy allow the user to selectively image layers in deep tissue at high speed. See “Random depth-access light-sheet imaging”

 

Faster 3D imaging with Bessel two-photon dual-projection imaging

Dual-projection volumetric microscopy supports 3D imaging in tissue at 100 VPS. Combining with two-photon glutamate uncaging, the microscopy technique enables studying neuron circuit functions at high speed and high spatial resolution. See “Calcium in neural tissue under uncaging stimulation” page for our recent results.