Bessel Light Sheet Two-Photon Imaging
Zebrafish movies
kdrl:GFP Zebrafish
3D movie of the entire zebrafish head at 3dpf. Vessels are labeled with GFP.
Video rate movie of heart beat in live zebrafish embryo. Vessels are labeled with GFP.
pod:NTR-mCherry; kdrl:GFP double transgenic Zebrafish
Kidney structure at 5dpf. GFP(green) labels endothelial cells in the vasculature. mCherry (red) labels podocytes. Kidney in 5dpf fish locates beneath 200 μm tissue.
Movie 1: Blurry structure seen with plane illumination
Movie 2: Clear cellular structure seen with NSIM scatter removal
———————————–
Mouse brain movies
Thy1:YFP Mouse brain
Fly through movie of 3D image, obtained with plain light sheet at 100 fps
Fly through movie of high clarity 3D image, obtained with the NSIM scatter removal method
3D rendered high clarity 3D image, obtained with the NSIM scatter removal method
GcAMP expressing astrocyte
Time-lapse movie showing spontaneous activities. The movie was taken at 67fps (3.3 vps) and lasted more than 4 minutes.