Bessel Light Sheet Two-Photon Imaging

Zebrafish movies

kdrl:GFP Zebrafish

3D movie of the entire zebrafish head at 3dpf. Vessels are labeled with GFP.

Video rate movie of heart beat in live zebrafish embryo. Vessels are labeled with GFP.

 

pod:NTR-mCherry; kdrl:GFP double transgenic Zebrafish

Kidney structure at 5dpf. GFP(green) labels endothelial cells in the vasculature. mCherry (red) labels podocytes. Kidney in 5dpf fish locates beneath 200 μm tissue.

Movie 1: Blurry structure seen with plane illumination

Movie 2: Clear cellular structure seen with NSIM scatter removal

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Mouse brain movies

Thy1:YFP Mouse brain

Fly through movie of 3D image, obtained with plain light sheet at 100 fps

Fly through movie of high clarity 3D image, obtained with the NSIM scatter removal method

3D rendered high clarity 3D image, obtained with the NSIM scatter removal method

 

Calcium levels in GcAMP expressing astrocyte

Mean Calcium level during the time-lapse imaging period.

Time-lapse movie showing spontaneous activities. The movie was taken at  67fps (3.3 vps) and lasted more than 4 minutes.

 

 

Random depth-access light sheet imaging

Calcium in neural tissue under two-photon glutamate uncaging stimulation