Calcium in neural tissue under two-photon glutamate uncaging stimulation

 

Bessel 2photon light sheet imaging with 2photon glutamate uncaging

 

GCaMP8m expressed neuron. Vol size: 120 x 120 x 32 μm3
Light sheet imaging speed: 100Hz frame rate, 3 VPS
Uncaging laser: 10x 1ms pulses (50% duty cycle 50Hz), 720 nm near a dendrite spine.

Bessel 2photon dual-projection imaging with 2photon glutamate uncaging

 

Raw movie:

GCaMP8m expressed neuron Ca2+ imaging. Vol size: 120 x 120 x 40 μm3
Volumetric speed: 100 VPS. Recording time: 30 sec
Uncaging laser: 10x pulses (50% duty cycle 50Hz), 720 nm near a dendrite spine

3D structure of the neuron extracted from the dual projection movie:

Calcium activities in selected location:

 

Manuscript under preparation