{"id":19,"date":"2017-03-08T15:26:26","date_gmt":"2017-03-08T22:26:26","guid":{"rendered":"https:\/\/live-optics-wp.pantheonsite.io\/lpeng\/?page_id=19"},"modified":"2025-02-15T13:28:29","modified_gmt":"2025-02-15T20:28:29","slug":"lightsheet2p","status":"publish","type":"page","link":"https:\/\/wp.optics.arizona.edu\/lpeng\/lightsheet2p\/","title":{"rendered":"Bessel Light Sheet Two-Photon Imaging"},"content":{"rendered":"<h2><span style=\"font-size: 14pt\">Zebrafish <\/span><span style=\"font-size: 14pt\">movies<\/span><\/h2>\n<p><strong><u><i>kdrl:GFP Zebrafish<\/i><\/u><\/strong><\/p>\n<div style=\"width: 860px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-19-1\" width=\"860\" height=\"511\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/SupMovie1_Head_20130713.mp4?_=1\" \/><a href=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/SupMovie1_Head_20130713.mp4\">https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/SupMovie1_Head_20130713.mp4<\/a><\/video><\/div>\n<p>3D movie of the entire zebrafish head at 3dpf. Vessels are labeled with GFP.<\/p>\n<div style=\"width: 512px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-19-2\" width=\"512\" height=\"512\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/SupMovie3_Heartbeat20130809.mp4?_=2\" \/><a href=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/SupMovie3_Heartbeat20130809.mp4\">https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/SupMovie3_Heartbeat20130809.mp4<\/a><\/video><\/div>\n<p>Video rate movie of heart beat in live zebrafish embryo.\u00a0Vessels are labeled with GFP.<\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"text-decoration: underline\"><strong><em>pod:NTR-mCherry; kdrl:GFP double transgenic Zebrafish<\/em><\/strong><\/span><\/p>\n<p>Kidney structure at 5dpf. GFP(green) labels\u00a0endothelial cells in the vasculature.\u00a0mCherry (red) labels\u00a0podocytes. Kidney in 5dpf fish locates beneath 200 \u03bcm tissue.<\/p>\n<div style=\"width: 606px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-19-3\" width=\"606\" height=\"350\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/SupMovie5_Kidney_0th.mp4?_=3\" \/><a href=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/SupMovie5_Kidney_0th.mp4\">https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/SupMovie5_Kidney_0th.mp4<\/a><\/video><\/div>\n<p>Movie 1: Blurry structure seen with plane illumination<\/p>\n<div style=\"width: 606px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-19-4\" width=\"606\" height=\"350\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/SupMovie6_Kidney_SIM.mp4?_=4\" \/><a href=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/SupMovie6_Kidney_SIM.mp4\">https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/SupMovie6_Kidney_SIM.mp4<\/a><\/video><\/div>\n<p>Movie 2: Clear cellular structure seen with NSIM scatter removal<\/p>\n<p>&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8212;&#8211;<\/p>\n<h2><span style=\"font-size: 14pt\">Mouse brain movies<\/span><\/h2>\n<p><strong><span style=\"text-decoration: underline\"><em>Thy1:YFP Mouse brain<\/em><\/span><\/strong><\/p>\n<div style=\"width: 860px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-19-5\" width=\"860\" height=\"538\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/XYFly_Plane.mp4?_=5\" \/><a href=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/XYFly_Plane.mp4\">https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/XYFly_Plane.mp4<\/a><\/video><\/div>\n<p>Fly through movie of 3D image, obtained with plain light sheet at 100 fps<\/p>\n<div style=\"width: 860px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-19-6\" width=\"860\" height=\"538\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/XYFly_SIM.mp4?_=6\" \/><a href=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/XYFly_SIM.mp4\">https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/XYFly_SIM.mp4<\/a><\/video><\/div>\n<p>Fly through movie of high clarity 3D image, obtained with the NSIM scatter removal method<\/p>\n<div style=\"width: 860px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-19-7\" width=\"860\" height=\"860\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/Rot45_3D_SIM.mp4?_=7\" \/><a href=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/Rot45_3D_SIM.mp4\">https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/Rot45_3D_SIM.mp4<\/a><\/video><\/div>\n<p>3D rendered\u00a0high clarity 3D image, obtained with the NSIM scatter removal method<\/p>\n<p>&nbsp;<\/p>\n<p><em><span style=\"text-decoration: underline\"><strong>Calcium levels in GcAMP expressing astrocyte<\/strong><\/span><\/em><\/p>\n<div style=\"width: 826px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-19-8\" width=\"826\" height=\"826\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/MeanStack3D.mp4?_=8\" \/><a href=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/MeanStack3D.mp4\">https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/MeanStack3D.mp4<\/a><\/video><\/div>\n<p>Mean Calcium level during the time-lapse imaging period.<\/p>\n<div style=\"width: 860px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-19-9\" width=\"860\" height=\"573\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/MultiViewTimelapse_Green2Purple.mp4?_=9\" \/><a href=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/MultiViewTimelapse_Green2Purple.mp4\">https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/MultiViewTimelapse_Green2Purple.mp4<\/a><\/video><\/div>\n<p>Time-lapse movie showing spontaneous activities. The movie was taken at \u00a067fps (3.3 vps) and lasted more than 4 minutes.<\/p>\n<p>&nbsp;<\/p>\n<p><!--more--><\/p>\n<p>&nbsp;<\/p>\n<p><a href=\"https:\/\/wp.optics.arizona.edu\/lpeng\/ra_lightsheet\/\"><em><span style=\"text-decoration: underline\"><strong>Random depth-access light sheet imaging<\/strong><\/span><\/em><\/a><\/p>\n<p><a href=\"https:\/\/wp.optics.arizona.edu\/lpeng\/calcium-in-neural-tissue-under-uncaging-stimulation\/\"><em><span style=\"text-decoration: underline\"><strong>Calcium in neural tissue under two-photon glutamate uncaging stimulation<\/strong><\/span><\/em><\/a><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Zebrafish movies kdrl:GFP Zebrafish 3D movie of the entire zebrafish head at 3dpf. Vessels are labeled with GFP. Video rate movie of heart beat in live zebrafish embryo.\u00a0Vessels are labeled with GFP. &nbsp; pod:NTR-mCherry; kdrl:GFP double transgenic Zebrafish Kidney structure at 5dpf. GFP(green) labels\u00a0endothelial cells in the vasculature.\u00a0mCherry (red) labels\u00a0podocytes. Kidney in 5dpf fish locates beneath 200 \u03bcm tissue. Movie<\/p>\n","protected":false},"author":3,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-19","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-json\/wp\/v2\/pages\/19","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-json\/wp\/v2\/comments?post=19"}],"version-history":[{"count":43,"href":"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-json\/wp\/v2\/pages\/19\/revisions"}],"predecessor-version":[{"id":158,"href":"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-json\/wp\/v2\/pages\/19\/revisions\/158"}],"wp:attachment":[{"href":"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-json\/wp\/v2\/media?parent=19"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}