{"id":115,"date":"2025-02-15T11:19:52","date_gmt":"2025-02-15T18:19:52","guid":{"rendered":"https:\/\/live-optics-wp.pantheonsite.io\/lpeng\/?page_id=115"},"modified":"2026-04-14T18:26:05","modified_gmt":"2026-04-15T01:26:05","slug":"calcium-in-neural-tissue-under-uncaging-stimulation","status":"publish","type":"page","link":"https:\/\/wp.optics.arizona.edu\/lpeng\/calcium-in-neural-tissue-under-uncaging-stimulation\/","title":{"rendered":"Calcium in neural tissue under two-photon glutamate uncaging stimulation"},"content":{"rendered":"<p>&nbsp;<\/p>\n<h5><b><i><u>Bessel 2photon light sheet imaging with 2photon glutamate uncaging<\/u><\/i><\/b><\/h5>\n<p>&nbsp;<\/p>\n<div style=\"width: 860px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-115-1\" width=\"860\" height=\"382\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/Uncaging3Drendering_v2.mp4?_=1\" \/><a href=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/Uncaging3Drendering_v2.mp4\">https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/Uncaging3Drendering_v2.mp4<\/a><\/video><\/div>\n<p>GCaMP8m expressed neuron. Vol size: 120 x 120 x 32 \u03bcm3<br \/>\nLight sheet imaging speed: 100Hz frame rate, 3 VPS<br \/>\nUncaging laser: 10x 1ms pulses (50% duty cycle 50Hz), 720 nm near a dendrite spine.<\/p>\n<h2><\/h2>\n<h5><b><i><u>Bessel 2photon dual-projection imaging with 2photon glutamate uncaging<\/u><\/i><\/b><\/h5>\n<p>&nbsp;<\/p>\n<p><strong>Raw movie:<\/strong><\/p>\n<div style=\"width: 860px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-115-2\" width=\"860\" height=\"382\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/Projections.mp4?_=2\" \/><a href=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/Projections.mp4\">https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/Projections.mp4<\/a><\/video><\/div>\n<p>GCaMP8m expressed neuron Ca2+ imaging. Vol size: 120 x 120 x 40 \u03bcm3<br \/>\n<strong>Volumetric speed: 100 VPS.<\/strong> Recording time: 30 sec<br \/>\nUncaging laser: 10x pulses (50% duty cycle 50Hz), 720 nm near a dendrite spine<\/p>\n<p><strong>3D structure of the neuron extracted from the dual projection movie:<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-106\" src=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/StructSec_2pProj-300x168.jpg\" alt=\"\" width=\"464\" height=\"260\" srcset=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/StructSec_2pProj-300x168.jpg 300w, https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/StructSec_2pProj-1024x572.jpg 1024w, https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/StructSec_2pProj-768x429.jpg 768w, https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/StructSec_2pProj-1536x858.jpg 1536w, https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/StructSec_2pProj.jpg 1760w\" sizes=\"auto, (max-width: 464px) 100vw, 464px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-104\" src=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/Struct_2pProj-300x153.jpg\" alt=\"\" width=\"467\" height=\"238\" srcset=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/Struct_2pProj-300x153.jpg 300w, https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/Struct_2pProj-1024x521.jpg 1024w, https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/Struct_2pProj-768x391.jpg 768w, https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/Struct_2pProj-1536x782.jpg 1536w, https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/Struct_2pProj.jpg 1745w\" sizes=\"auto, (max-width: 467px) 100vw, 467px\" \/><\/p>\n<p><strong>Calcium activities in selected location:<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-107\" src=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/Activity_2pProj-300x103.jpg\" alt=\"\" width=\"699\" height=\"240\" srcset=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/Activity_2pProj-300x103.jpg 300w, https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/Activity_2pProj-1024x352.jpg 1024w, https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/Activity_2pProj-768x264.jpg 768w, https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/Activity_2pProj-1536x529.jpg 1536w, https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/Activity_2pProj-2048x705.jpg 2048w\" sizes=\"auto, (max-width: 699px) 100vw, 699px\" \/><\/p>\n<p>&nbsp;<\/p>\n<p>Manuscript under review<\/p>\n","protected":false},"excerpt":{"rendered":"<p>&nbsp; Bessel 2photon light sheet imaging with 2photon glutamate uncaging &nbsp; GCaMP8m expressed neuron. Vol size: 120 x 120 x 32 \u03bcm3 Light sheet imaging speed: 100Hz frame rate, 3 VPS Uncaging laser: 10x 1ms pulses (50% duty cycle 50Hz), 720 nm near a dendrite spine. Bessel 2photon dual-projection imaging with 2photon glutamate uncaging &nbsp; Raw movie: GCaMP8m expressed neuron<\/p>\n","protected":false},"author":80,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-115","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-json\/wp\/v2\/pages\/115","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-json\/wp\/v2\/users\/80"}],"replies":[{"embeddable":true,"href":"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-json\/wp\/v2\/comments?post=115"}],"version-history":[{"count":7,"href":"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-json\/wp\/v2\/pages\/115\/revisions"}],"predecessor-version":[{"id":169,"href":"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-json\/wp\/v2\/pages\/115\/revisions\/169"}],"wp:attachment":[{"href":"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-json\/wp\/v2\/media?parent=115"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}