{"id":115,"date":"2025-02-15T11:19:52","date_gmt":"2025-02-15T18:19:52","guid":{"rendered":"https:\/\/live-optics-wp.pantheonsite.io\/lpeng\/?page_id=115"},"modified":"2026-09-14T14:31:22","modified_gmt":"2026-09-14T21:31:22","slug":"calcium-in-neural-tissue-under-uncaging-stimulation","status":"publish","type":"page","link":"https:\/\/wp.optics.arizona.edu\/lpeng\/calcium-in-neural-tissue-under-uncaging-stimulation\/","title":{"rendered":"Calcium in neural tissue under two-photon glutamate uncaging stimulation"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\">&nbsp;<\/p>\n\n\n\n<h5 class=\"wp-block-heading\"><b><i><u>Bessel 2photon light sheet imaging with 2photon glutamate uncaging<\/u><\/i><\/b><\/h5>\n\n\n\n<p class=\"wp-block-paragraph\">&nbsp;<\/p>\n\n\n\n<figure class=\"wp-block-video\"><video controls src=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/Uncaging3Drendering_v2.mp4\"><\/video><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\">GCaMP8m expressed neuron. Vol size: 120 x 120 x 32 \u03bcm3<br>Light sheet imaging speed: 100Hz frame rate, 3 VPS<br>Uncaging laser: 10x 1ms pulses (50% duty cycle 50Hz), 720 nm near a dendrite spine.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">&nbsp;<\/h2>\n\n\n\n<h5 class=\"wp-block-heading\"><b><i><u>Bessel 2photon dual-projection imaging with 2photon glutamate uncaging<\/u><\/i><\/b><\/h5>\n\n\n\n<p class=\"wp-block-paragraph\">&nbsp;<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Raw movie:<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-video\"><video controls src=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2025\/02\/Projections.mp4\"><\/video><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\">GCaMP8m expressed neuron Ca2+ imaging. Vol size: 120 x 120 x 40 \u03bcm3<br><strong>Volumetric speed: 100 VPS.<\/strong> Recording time: 30 sec<br>Uncaging laser: 10x pulses (50% duty cycle 50Hz), 720 nm near a dendrite spine<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>3D structure of the neuron extracted from the dual projection movie:<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">&nbsp;<\/p>\n\n\n\n<figure class=\"wp-block-image size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"762\" src=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2026\/09\/Raw-1024x762.jpg\" alt=\"\" class=\"wp-image-183\" style=\"aspect-ratio:1.3438399471523617;width:519px;height:auto\" srcset=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2026\/09\/Raw-1024x762.jpg 1024w, https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2026\/09\/Raw-300x223.jpg 300w, https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2026\/09\/Raw-768x572.jpg 768w, https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2026\/09\/Raw.jpg 1247w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\">\u00a0<\/p>\n\n\n\n<figure class=\"wp-block-image size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"510\" src=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2026\/09\/Struct3D-1024x510.jpg\" alt=\"\" class=\"wp-image-186\" style=\"aspect-ratio:2.0078962210941906;width:510px;height:auto\" srcset=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2026\/09\/Struct3D-1024x510.jpg 1024w, https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2026\/09\/Struct3D-300x149.jpg 300w, https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2026\/09\/Struct3D-768x382.jpg 768w, https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2026\/09\/Struct3D-420x210.jpg 420w, https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2026\/09\/Struct3D.jpg 1300w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Calcium activities in selected location:<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-image size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"101\" src=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2026\/09\/NeuronStructure-1024x101.jpg\" alt=\"\" class=\"wp-image-179\" style=\"aspect-ratio:10.13883944464222;width:562px;height:auto\" srcset=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2026\/09\/NeuronStructure-1024x101.jpg 1024w, https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2026\/09\/NeuronStructure-300x30.jpg 300w, https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2026\/09\/NeuronStructure-768x76.jpg 768w, https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2026\/09\/NeuronStructure-1536x152.jpg 1536w, https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2026\/09\/NeuronStructure-2048x202.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"879\" height=\"1024\" src=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2026\/09\/Sig-879x1024.jpg\" alt=\"\" class=\"wp-image-181\" style=\"width:566px;height:auto\" srcset=\"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2026\/09\/Sig-879x1024.jpg 879w, https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2026\/09\/Sig-257x300.jpg 257w, https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2026\/09\/Sig-768x895.jpg 768w, https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2026\/09\/Sig-1318x1536.jpg 1318w, https:\/\/wp.optics.arizona.edu\/lpeng\/wp-content\/uploads\/sites\/64\/2026\/09\/Sig-1758x2048.jpg 1758w\" sizes=\"auto, (max-width: 879px) 100vw, 879px\" \/><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\">Xu, D., Hwang, FJ., Ding, J.B. <i>et al.<\/i> Two-photon 3D imaging of optically stimulated neural activity at 100\u2009Hz. <i>Light Sci Appl<\/i> <b>15<\/b>, 303 (2026). https:\/\/doi.org\/10.1038\/s41377-026-02395-2<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n","protected":false},"excerpt":{"rendered":"<p>&nbsp; Bessel 2photon light sheet imaging with 2photon glutamate uncaging &nbsp; GCaMP8m expressed neuron. Vol size: 120 x 120 x 32 \u03bcm3Light sheet imaging speed: 100Hz frame rate, 3 VPSUncaging laser: 10x 1ms pulses (50% duty cycle 50Hz), 720 nm near a dendrite spine. &nbsp; Bessel 2photon dual-projection imaging with 2photon glutamate uncaging &nbsp; Raw movie: GCaMP8m expressed neuron Ca2+<\/p>\n","protected":false},"author":80,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-115","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-json\/wp\/v2\/pages\/115","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-json\/wp\/v2\/users\/80"}],"replies":[{"embeddable":true,"href":"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-json\/wp\/v2\/comments?post=115"}],"version-history":[{"count":11,"href":"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-json\/wp\/v2\/pages\/115\/revisions"}],"predecessor-version":[{"id":189,"href":"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-json\/wp\/v2\/pages\/115\/revisions\/189"}],"wp:attachment":[{"href":"https:\/\/wp.optics.arizona.edu\/lpeng\/wp-json\/wp\/v2\/media?parent=115"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}